Figure 7

The closed-open conformational equilibrium of the LOV core (top). The equilibrium is shifted toward the closed conformation in the dark state and toward the open conformation in the presence of BL. Hp indicates the hydrophobic region in the β-sheet. In AUREO1 (bottom), the closed conformation is stabilized by intramolecular hydrophobic interactions involving L201, L208, and L212 in the A'α helix and F219, V314, and V316 in the β-sheet. BL shifts the equilibrium of the LOV core toward the open conformation and deforms the hydrophobic surface of the β-sheet containing F219, V314, and V316, subsequently inducing the detachment of the A'α helix and the dimerization of AUREO1. The AUREO1 dimer is stabilized by intermolecular hydrophobic interactions involving F219, F299, L303, V314, and V316.