Figure 4 | Scientific Reports

Figure 4

From: Osteogenic differentiation of human mesenchymal stromal cells and fibroblasts differs depending on tissue origin and replicative senescence

Figure 4

ALK-6 expression in P3 versus culture senescence cells at P10. (A) Evaluated was the ALK-6 expression in DSC at P3. Expression of ALK-6 increased over the course of osteogenic differentiation. (B) Evaluated was the ALK-6 expression in DSC at P10. (C) Evaluated was the ALK-6 expression in ASC at P3. (D) Evaluated was the ALK-6 expression in ASC in P10. ALK-6 was expressed at a small amount. (E) Evaluated was the ALK-6 expression in FB in P3. The expression increased weakly between day 7 and 14. On day 7 was a significant difference between the BMP-2 treatment and the standard osteogenic differentiation in favor to the BMP-2 supplementation. (F) Evaluated was the ALK-6 expression in FB at P10. Grey bars represent these cells incubated with osteogenic differentiation media supplemented with BMP-2 (OM + BMP2). White bars represent cells differentiated with standard osteogenic differentiation media (OM). Bars represent mean ± SD of three donors. *p < 0.05 as compared to the respective sample treated with OM.

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