Figure 8 | Scientific Reports

Figure 8

From: Osteogenic differentiation of human mesenchymal stromal cells and fibroblasts differs depending on tissue origin and replicative senescence

Figure 8

Evaluation of senescence associated β-galactosidase activity. (A) Visualized comparison of β-galactosidase activity at P3 and P10. At P3 in DSC, ASC and FB β-galactosidase activity was on a comparable level. Control cells treated with etoposide, showed an increase of β-galactosidase activity. At P10 DSC and FB showed a noticeable stronger β-galactosidase staining compared to ASC. Shown are representative illustrations of at least six identical results. The image scales represent a length of 100 µm. (B) Evaluation β-galactosidase expression at P3 and P10. In ASC β-galactosidase expression was on a comparable level in P3 and P10. In DSC and FB the β-galactosidase expression noticeably increased in P10 in contrast to P3. Bars represent mean ± SD of two donors.

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