Figure 1
From: Comparison of the sensitivity of Western blotting between PVDF and NC membranes
Comparison of the binding ability of PVDF membrane and NC membrane to low molecular weight proteins. (a) Indicated numerals are amounts (3.0, 1.5, 0.8, 0.4, 0.2 and 0.1 μg) of the pooled sera proteins subjected to 8% SDS-PAGE or cell proteins (40, 20, 10, 5 and 2.5 μg) subjected to 12% SDS-PAGE. The electroblotted membranes are PVDF membrane (top) and NC membrane (bottom), respectively. The membranes were incubated with anti-IgG, anti-ApoAl and anti-BCL2 antibodies. (b) Staining intensities were statistically analyzed (n = 3 individual experiments). Pink bar, PVDF membrane; Blue bar, NC membrane. Band intensities were analyzed and compared using Image Lab software (Bio-Rad Laboratories) and GraphPad Prism version 6. *Significantly different p < 0.05, **p < 0.01, ***p < 0.001. N.S., not significant. All values are means ± S.E. (error bars).
