Figure 2
From: Comparison of the sensitivity of Western blotting between PVDF and NC membranes
Comparison of the binding ability of PVDF membrane and NC membrane to medium molecular weight proteins. (a) The pooled sera proteins (0.1–3.0 μg) were subjected to 8% SDS-PAGE. The electroblotted membranes are PVDF membrane (up) and NC membrane (down), respectively. The membranes were incubated with anti-alpha-1-acid glycoprotein (AGP), anti-eukaryotic transformation extension factor 1 alpha 2 (EEF1A2) and anti-transferrin (TF) antibodies. (b) Staining intensities were statistically analyzed (n = 3 individual experiments). Pink bar, PVDF membrane; Blue bar, NC membrane. Band intensities were analyzed and compared using Image Lab software (Bio-Rad Laboratories) and GraphPad Prism version 6. N.S., not significant. All values are means ± S.E. (error bars).
