Figure 5
From: Comparison of the sensitivity of Western blotting between PVDF and NC membranes
Comparison of the re-probed ability of PVDF membrane and NC membrane. The pooled sera proteins (3.0, 1.5, 0.7, 0.3 and 0.1 μg) were separated by 8% SDS-PAGE, and transferred to PVDF membranes (up) and NC membrane (down), respectively. (a) Staining with AAL and then re-probed with PHA-E; (b) staining with ApoA1 and then re-probing with IgG; (c) Staining with PHA-E and then re-probing with A2M; (d) staining with A2M and then re-probing with PHA-E. Band intensities were statistically analyzed (n = 3 individual experiments) and compared using Image Lab software (Bio-Rad Laboratories) and GraphPad Prism version 6. Pink bar, PVDF membrane; Blue bar, NC membrane. Band intensities were analyzed *Significantly different p < 0.05, **p < 0.01. N.S., not significant. All values are means ± S.E. (error bars).
