Figure 6
From: Comparison of the sensitivity of Western blotting between PVDF and NC membranes
Comparison of the binding ability of PVDF membrane and NC membrane to trypsin, fetuin, BSA, lactase. PVDF and NC membranes (0.5 cm × 0.5 cm) were incubated with different concentrations (40, 80, 160, 320, 640, 1280, 2560 ug/500 uL) of proteins, such as (a) trypsin, (b) fetuin, (c) BSA, and lactase (d) for 2 h, followed with DB-71 staining. The left side of a, b, c, and d: representative blots; The right side of (a–d) the relative signal intensity of the proteins that binded on the two membranes were detected by staining with DB-71. Image J and GraphPad Prism Version 6 were used for density analysis and statistics. Pink line, PVDF membrane; Blue line, NC membrane. *Significantly different p < 0.05, **p < 0.01, ***p < 0.001. N.S., not significant. All values are means ± S.E. (error bars).
