Table 2 Antibodies, lectins and proteins, as well as their molecular weights, transfer time and incubate time.
From: Comparison of the sensitivity of Western blotting between PVDF and NC membranes
Antibody | Molecular weight (kDa) | Transfer time (min) |
|---|---|---|
(a) Low molecular weight proteins | ||
IgG | 50, 25 | 60 |
ApoA1 | 27 | 60 |
BCL2 | 30 | 50 |
HBB | 15 | 50 |
(b) Medium molecular weight proteins | ||
AGP | 50 | 60 |
EEF1A2 | 50 | 70 |
TF | 80 | 60 |
(c) High molecular weight proteins | ||
CerP | 150 | 90 |
Non-reduced IgG | 150 | 60 |
A2M | 170 | 60 |
(d) Lectins | ||
AAL | – | 60 |
PHA-E | – | 60 |
SNA | – | 60 |
(e) Proteins | ||
Trypsin | 24 | 120 |
Fetuin | 40 | 120 |
BSA | 66 | 120 |
Lactase | 130 | 120 |
