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Figure 1

From: Anthrax toxin translocation complex reveals insight into the lethal factor unfolding and refolding mechanism

Figure 1The alternative text for this image may have been generated using AI.

Anthrax toxin immobilization, translocation, and nanodisc stabilization (TITaNS) method (A) PApore side view slice with funnel shape from α clamp (yellow) to Φ clamp (orange) and charge clamp (red) inside pore β barrel channel indicated. (B) Immobilization of LFN (magenta) PAprepore (blue) complexes on thiol sepharose beads (grey surface). (C) PAprepore transitioned to PApore. (D) Predicted translocation complex of LFN -PApore at low pH. (E) Addition of pre-nanodisc micelle (green) to complex. (F) Nanodisc formation. (G) LFN-PApore-Nanodisc translocation complexes at pH 5.5 on cryoEM grid.

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