Figure 3
Unfolding and refolding of LFN at key clamp sites during translocation through PApore. (A) Model of PApore (blue) translocating LFN (magenta) with α clamp, Φ clamp, and charge clamp residues shown in yellow, orange, and red respectively. CryoEM density of LFN shown in transparent grey. (B) Hydrophobic residues predicted to facilitate unfolding in early translocation from α clamp to Φ clamp. (C) Φ clamp ring with LFN density. (D) F427 residues for each subunit with associated cryoEM density compared to modelled density. (E) LFN model and density in β barrel charge clamp. (F) PApore channel exit into the cytosol with LFN α1 helical model and cryoEM density. (G) 90° rotation of F. Discontinuous density indicated in model with dashed magenta lines.
