Figure 6
CRF Deletion in BNST Selectively Alters Sensory-Discriminative and Affective-Motivational Behaviors. (A) Schematic of hot plate test. (B) Thermal nociceptive sensitivity of male (n = 16–17) and female (n = 15–17) Floxed-CRF mice, as defined by latency to first paw withdrawal for each mouse (Two-way ANOVA with Tukey’s post hoc: no Sex × Virus interaction [F(1, 61) = 1.853, P = 0.1785] or main effect of Sex [F(1, 61) = 0.1124, P = 0.7386] and Virus [F(1, 61) = 0.1524, P = 0.6976]). (C) Quantification of sensory-discriminative and affective-motivational behaviors throughout the 45-s exposure to hot plate. Averaged area under the curve (AUC) of paw withdrawal (Two-way ANOVA with Tukey’s post hoc: no Sex × Virus interaction [F(1, 61) = 1.072, P = 0.3047] or main effect of Sex [F(1, 61) = 1.885, P = 0.1747] and Virus [F(1, 61) = 0.6208, P = 0.4338]), paw attending (Two-way ANOVA with Tukey’s post hoc: no Sex × Virus interaction [F(1, 61) = 2.605, P = 0.1117] or main effect of Sex [F(1, 61) = 0.8298, P = 0.3659]; main effect of Virus [F(1, 61) = 4.695, P = 0.0342]), and paw guarding (Two-way ANOVA with Tukey’s post hoc: no Sex × Virus interaction [F(1, 61) = 0.09333, P = 0.7610] or main effect of Sex [F(1, 61) = 1.026, P = 0.3152] and Virus [F(1, 61) = 0.04448, P = 0.8337]) are shown, with corresponding cumulative distribution functions displayed in (D–E). (D–E) Sensory-discriminative and affective-motivational behaviors are exhibited across time with cumulative distribution functions for (D) male and (E) female subjects (paw withdrawal: CON (M): 391.9 [341.0–442.9], CRE (M): 311.7 [263.2–360.2], CON (F): 285.9 [249.1–322.7], CRE (F): 296.8 [261.1–332.5]; paw attending: CON (M): 11.00 [4.672–17.33], CRE (M): 14.03 [6.087–21.97], CON (F): 7.147 [2.442–11.85], CRE (F): 27.87 [17.95–37.78]; paw guarding: CON (M): 189.3 [171.7–207.0], CRE (M): 179.3 [156.7–201.9], CON (F): 163.8 [147.6–180.0], CRE (F): 165.6 [147.8–183.4]). Data are shown as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001, ****P < 0.0001.
