Figure 2
From: Tanshinones induce tumor cell apoptosis via directly targeting FHIT
STS directly binds to FHIT protein. (A) Chemical structure of STS. (B) Cellular MST assay of the interaction between STS and FHIT. HEK293T cell lysate containing GFP tagged FHIT (black circle) or Red-NHS labeled FHIT (red square) were incubated with STS. The Kd value is 268.4 ± 42.59 nM and 1.82 ± 0.3 µM for GFP-FHIT and Red-NHS FHIT, respectively. (C) Schematic representation of the full-length FHIT and the truncated mutants used in the MST assay. The N-terminal region (1-108aa) containing the HIT domain is colored by blue, and the C terminal is colored by red. (D) MST assay of STS and GFP tagged FHIT truncations, the Kd value is 75.05 µM for TR1 (blue circle), and 58.18 µM for TR2 (blue square). No Kd value was calculated with TR3 (dull-red triangle). (E) Representation of molecule docking of FHIT-STS complex by Autodock vina software based on the FHIT-AMP complex (PDB code:3FIT). The region of 1-43aa is shown in mazarine, 44-108aa is shown in wathet, 109-147aa is shown in red. STS is shown in green sticks and AMP is shown in purple sticks. Structure alignment indicated that STS almost occupies the AMP binding site. (F) The surface model of the predicated FHIT-STS complex.
