Figure 4 | Scientific Reports

Figure 4

From: Gender specific airway gene expression in COPD sub-phenotypes supports a role of mitochondria and of different types of leukocytes

Figure 4

Molecular analysis of MT-CO2 in COPD sub-phenotypes. (A) Using genomic DNA for PCR, data of tested gene (MT-CO2 and SDHA) and housekeeping gene (alpha-enolase) were processed with the same settings (e.g. same thresholds). Cycle number of the housekeeping gene was subtracted from the corresponding gene and its absolute value was subsequently calculated to the power of 2. ct = cycle number at threshold; MT-CO2/SDHA ratio Aex versus Eex p = 0.33 (Mann Whitney U-Test). The average level for MT-CO2 transcripts was 8255 ± 2938 cpm for Aex samples and 30,902 ± 10,200 cpm for Eex samples (B) MT-CO2 expression levels were determined by RT-PCR using primers given in Table S7 on brush samples and on whole blood samples from the same individuals. Expression levels were normalized to the levels of alpha-enolase. Samples 1–4 are Eex cases, samples 5–7 are Aex cases.

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