Figure 3

FSEC and hFSEC analysis of AtBOR1 proteins. FSEC analysis is carried out on protein solubilised at 4 °C prior to separation on the SEC column and hFSEC analysis is carried out on protein initially prepared in the same way then heated at 46 °C for 10 min, prior to separation on the SEC column. In each case, aggregated material elutes at roughly fraction 8, with monodispersed protein eluting at roughly fraction 35, labelled A and M respectively on (A). (A–C) Show the results obtained for D311A, P362G and S466R variants compared to WT AtBOR1 analysed at the same time, respectively. In each case the elution trace obtained for unheated sample is shown in the solid line while the elution trace obtained for heated sample is shown in the same coloured dashed line. Data is representative of at least n = 2 independent experiments.