Figure 1 | Scientific Reports

Figure 1

From: Tailored generation of insulin producing cells from canine mesenchymal stem cells derived from bone marrow and adipose tissue

Figure 1

cBM-MSC and cAD-MSC characterization. Morphological appearances of cBM-MSCs (A,B) and cAD-MSCs (H,I) were observed under phase-contrast microscope with magnification of 40X and 200X. mRNA expressions regarding stemness and proliferation markers (C,J) were determined by RT-qPCR. mRNA expression was normalized with a reference gene. The MSC-related surface markers were analyzed using flow cytometry (D,K). Osteogenic differentiation potential at day 14 post-induction was determined by Alizarin Red S and Von Kossa staining, and the osteogenic mRNA marker expression was analyzed by RT-qPCR (E,L). Chondrogenic differentiation potential at day 21 post-induction was determined by Alcian blue staining, and chondrogenic mRNA markers were determined (F,M). Adipogenic differentiation potential at day 28 post-induction was determined by Oil Red O staining, and adipogenic mRNA markers were determined (G,N). mRNA expression was normalized with a reference gene and undifferentiated control. Bars indicate a significant difference (*, p value < 0.05).

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