Figure 6

Fitness profile showing genes whose deletion strains are sensitive to treatment by metabolic intermediates of nitrosamines. Fitness defect scores are plotted on the vertical axis vs genes on the horizontal axis. Strains affected by the compounds lack the following genes; methylamine: BCK1, DAL81, ARG3, FPS1 and GRR1. Ethylamine: DAL81, ARG3, SNF6, ELO3, SNF6 and ELO3. Formamide: ARG3, FPS1, NPL6 and SPT4. Ammonium sulfate: DAL81, ARG3, PEP3, THR1, etc. It could be seen that ARG3 was affected by all the metabolites, in addition to ARG1 being affected by methylamine and formamide. Concentration and date of count matrices analysis are indicated. Not all strains with significant FD scores are labelled. Plots were generated using the Bioconductor v3.12, https://www.bioconductor.org/.