Figure 9

Gene ontology enrichment profiles showing pathways affected by the metabolic intermediates of N-nitrosamines. Genes deleted in strains that displayed increased sensitivity were enriched for certain biological processes including; methylamine include: chromatin remodeling, protein targeting to vacuole, microautophagy and arginine biosynthetic process. Ethylamine: cell wall chitin biosynthetic process and conjugation with cellular fusion. Formamide: arginine biosynthetic process, chromatin remodeling, ATP export and protein transport. Ammonium sulfate: protein targeting to vacuole, phosphorylation and cellular amino acid biosynthetic process. Enrichment profiles were generated using the Bioconductor v3.12, https://www.bioconductor.org/.