Figure 2

Cumulative CH4 production by M. barkeri strain MS (from left to right) in media without perchlorate (Control), Ca(ClO4), Mg(ClO4)2, or Na(ClO4) at 30 °C (filled triangles) and 0 °C (open triangles). Statistically significant differences in CH4 production in perchlorate-supplemented incubations relative to the control of the same temperature are indicated by asterisks (*) (paired t-test, P < 0.05).