Figure 3
Statistically significant differential expression (Log2-fold change, LFC; Wald test, P < 0.05) of methanogenesis genes in M. barkeri strain MS. Differential expression of each gene across 7 assessed conditions is indicated via heatmap. Averages are presented for multi-copy genes (for exact values, reference Tables 1 and 2). Differential expression of genes in 0 °C perchlorate-amended transcriptomes are relative to the expression patterns of the 0 °C perchlorate-free control. Perchlorate-amended 30 °C and 0 °C perchlorate-free control transcriptomes are relative to the expression patterns of the 30 °C perchlorate-free control. Crossed white boxes indicate no statistically significant difference in expression. Heatmaps are excluded from genes exhibiting no significant differential expression across all conditions. Genes involved in non-hydrogenotrophic (H2/CO2) pathways are encompassed in colored boxes with pathway directionality indicated by matching arrowheads (green: methylamines; pink: acetate; yellow: methanol). Full names of listed genes and metabolites are found in Tables S13 and S14, respectively.
