Figure 2 | Scientific Reports

Figure 2

From: Mutant p53s and chromosome 19 microRNA cluster overexpression regulate cancer testis antigen expression and cellular transformation in hepatocellular carcinoma

Figure 2

Overexpression of C19MC, AFP and zinc efflux pump RNAs are restricted to p53 transcription incompetent HCCs and miR-520G promotes AFP mRNA expression. (a) LIHC (HCC) classification of p53TC, p53TI tumors based on 10 p53-repressed genes and 20 p53-induced genes. Two large clusters that show clear differences were chosen. (b) HCCs showing overexpression of C19MC miRNAs in p53-TI cluster. (c) Heatmap of 31 genes showing differences between p53TC and p53TI clusters out of 200 cell death regulatory genes examined. (d) Overexpression of AFP in p53TI cluster (e) 324 SLC family gene heatmap showing a massive panel of SLC family members differ between p53TC and p53TI clusters. Major zinc-related SLCs are shown in box-whisker plots. (f) RNA-seq heatmap showing that, AFP, and zinc transporters/efflux pumps are promoted by C19MC miRNAs in stably transfected Hep3B cells. (g) RT-PCR showing C19MC miRNA regulation of AFP and AFP-transcription factors in Hep3B miRNA overexpressed Hep3B stable cells. Relative AFP mRNA expression quantified by densitometry is shown as bar graph in bottom panel.

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