Figure 5 | Scientific Reports

Figure 5

From: Mutant p53s and chromosome 19 microRNA cluster overexpression regulate cancer testis antigen expression and cellular transformation in hepatocellular carcinoma

Figure 5

Nickel and zinc induce miR-526B and definite cell death in Hep3B cells. (a) A map of Sp1 zinc (orange) and nickel (green) dependent DNA binding boxes within C19MC (grey box) and surrounding landscapes. 6-mer-to-10-mer palindromic sequences were mapped to a 200 kb region enclosing C19MC. (b–d) Nickel and zinc induce C19MC miRNA-526B expression in Hep3B cells as evaluated by qRT-PCR (panel-b), massive mitochondrial depolarization as evaluated by JC1 and Hoechst-33342 staining (pseudo-colored: panel-c) and block the expressions of MAGEA and other mRNAs while promoting endogenous defective p53 as evaluated by RT-PCR (panel-d). (e) Nickel and zinc combination induces definite cell death in Hep3B cells that are stably overexpressed with C19MC miRNAs. After 24 h of treatment, the pyknotic cells were washed and plated to evaluate survival.

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