Figure 7

IFN-γ co-operates with miR-520G to upregulate MAGEAs, MTF-1 and FOXM1. (a) 100 expressing ligand mRNAs (cytokines, chemokines and so on) out of 136 from TCGA-LIHC data (RNA-seq data integrated to miRNA-seq from patients who had high versus low cumulative C19MC miRNA expression) were correlated to C19MC miRNAs to pick the top positively correlated IFN-γ mRNA. Insignificant and no correlations were color coded as white. (b,c) A panel of indicated gene mRNAs were examined for expression changes in response to indicated cytokines along with IFN-γ at 24 h time point in Hep3B untransfected cells (Panel-b), and C19MC miRNA-520G stably transfected cells (Panel-c). Note the differences between panels b and c. (d) Wild-type or mutant p53s does not co-operate with miR-520G to change the expression of any of the indicated genes considerably except MTF-1. (e) Schematic representing the cell death and transformation signaling differences between p53TC and p53TI cells. The apoptotic/non-apoptotic cells and DNA were created using Lightwave-3D (https://www.newtek.com/lightwave/) v11.6.3. Rest of the image was created and composited in Adobe Photoshop CS5 (https://www.adobe.com/products/photoshopfamily/) v 12.0 × 64.