Figure 3
Heatmap of differentially expressed genes (DEGs) in the hypothalamus in each state (n = 2/time point/state except at hour 22 in photorefractory state where n = 1). DEGs (rows) were determined by comparing the expression value at hour 14, hour 18 or hour 22 with hour 10 value (column). Log-transformed values were normalized and plotted as z-scores (blue, minimum; red, maximum). A blue continuous line running through heatmap shows expression differences from mean value (dashed line). Genes with similar expression patterns have been clustered together. The right panel shows the results of significantly enriched pathways (padj. < 0.05), and STRING DEG network analysis in which the nodes/circles represent gene-encoded proteins, while the edge/lines connecting circles represent the protein–protein interaction. The empty nodes represent proteins of an unknown 3D structure, while the filled nodes represent the known or a predicted 3D structure.
