Figure 2

The BCGΔBCG1419c vaccine candidate is more attenuated than parental BCG. (a) Kinetics of murine macrophage infection assay in RAW 264.7 using BCG or BCG∆BCG1419c. Data (n = 6 per BCG strain/time point) is shown as mean with SD. A One-Way ANOVA followed by Tukey’s multiple comparison test or a Brown-Forsythe and Welch ANOVA followed by Dunnett’s multiple comparison test were used depending on SDs being or not different among groups compared, and significantly different p values indicated. (b) Body weight registry of individual athymic mice (n = 10/group) intravenously infected with 10⁶ CFU of BCG or BCGΔBCG1419c, used to inform humane endpoint (≥ 20% of weight loss). (c) Survival of athymic mice at 180 days post intravenous infection, with p value resulting from the Log-rank (Mantel-Cox) test shown. (d) Bacterial replication in lungs and spleen of athymic mice euthanized when they lost ≥ 20% of its maximum weight. Mean Log₁₀ CFU/organ with individual data (n = 10 per BCG strain) with SD are shown, and were compared with an unpaired, two-tailed Student’s t test, with Welch’s correction when standard deviation of the groups was different, with significantly different p values indicated. (e) Body weight registry of individual BALB/c mice (n = 10/group) subcutaneously vaccinated with 10⁷ CFU BCG or BCGΔBCG1419c over 2 months (63 days). (f) Body weight registry of individual C57BL/6 mice (n = 10/group) subcutaneously vaccinated with 10⁷ CFU BCG or BCGΔBCG1419c over 2 months (63 days). (g) Bacterial replication in lungs and spleen of BALB/c mice (n = 5 per time point) at 24 h and 2 months (63 days) post-vaccination. Mean Log₁₀ CFU/organ with individual data (n = 5 per BCG strain) with SD are shown, and were compared with an unpaired, two-tailed Student’s t test, with Welch´s correction when standard deviation of the groups was different, with significantly different p values indicated. (h) Bacterial replication in lungs and spleen of C57BL/6 mice (n = 5 per time point) at 24 h and 2 months (63 days) post-vaccination. Mean Log₁₀ CFU/organ with individual data (n = 5 per BCG strain) with SD are shown, and were compared with an unpaired, two-tailed Student’s t test, with Welch’s correction when standard deviation of the groups was different, with significantly different p values indicated.