Figure 4
From: Vaccination with BCGΔBCG1419c protects against pulmonary and extrapulmonary TB and is safer than BCG
The BCGΔBCG1419c vaccine candidate is more effective than BCG in reducing pulmonary TB in guinea pigs. (a) Bacterial replication in lungs of guinea pigs (n = 5 in saline and BCG groups, n = 10 in the BCGΔBCG1419c group) at 40 days post-infection. Mean Log10 CFU with SD are shown, including individual data. Brown-Forsythe and Welch ANOVA test followed by Dunnett’s multiple comparison was used to compare M. tuberculosis burden and p values are shown. (b) Representative H&E images (×2) of animals that received saline, BCG, or BCGΔBCG149c. Lesions were evaluated using a scoring system to compare them (c). Primary lesions. (d) Necrosis. (e) Total lung score. Data (n = 4–10, depending on groups) is presented as a box and whisker plot showing all points. Categorized data were analyzed with a H Kruskal–Wallis test using SPSS version 25 (α = 0.05). In all cases, p value was adjusted for multiple comparisons with Bonferroni correction. Secreted cytokines in lungs of infected guinea pigs at 40 days post-infection, showing (f) TNF-α, (g) IL-17, and (h) IL-10. Data (n = 4–10, depending on groups) is presented as a box and whisker plot showing all points. A One-Way ANOVA followed by Tukey’s multiple comparison test or a Kruskal–Wallis followed by Dunn’s multiple comparison test were used depending on distribution of data, and significantly different p values are indicated.
