Figure 3 | Scientific Reports

Figure 3

From: Identification of antigenic domains and peptides from VP15 of white spot syndrome virus and their antiviral effects in Marsupenaeus japonicus

Figure 3

Peptide design and evaluation of VP15-derived peptides in shrimp against WSSV. (A) De novo design of peptides using the VP15(26–57) amino acid sequence as a template. Four peptides, named KR11 (KTKSRRGSKKR), SR11 (STTAGRISKRR), SK10 (SPSMKKRAGK), and KK13 (KRRSPSMKKRAGK) were designed and chemically synthesized. (B) Time schedule of shrimp experiments using VP15-derived peptides as a protective agent. (C) Screening for the effective peptide by shrimp WSSV challenge experiment (KR11 (n = 13), SR11 (n = 11), SK10 (n = 11), or KK13 (n = 13)). Shrimp mortality was observed for 14 days, and the survival rate of each group was calculated. (D) Evaluation of SR11, an effective peptide, for enhancing shrimp survivability against WSSV. The experiment was conducted with twice the number of individuals (n = 25). For both experiments, VP15 (26–57) was used as a positive control, while PBS served as a negative control.

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