Table 2 Primers used in this study.
Name | Sequence (Direction from 5′ to 3′) |
|---|---|
pGEX-FW | GAAGTTCTGTTCCAGGGGCCC |
pGEX-RV | AGGCAGATCGTCAGTCAGTCA |
pFastBac-FW | TATTCCGGATTATTCATACC |
pFastBac-RV | ACAAATGTGGTATGGCTGATT |
pET41-RV | GGTTATGCTAGTTATTGCTC |
VP15(1–25) | |
pGEX-InvFW-Flag | GACTACAAGGATGACGATGACAAGTAAG |
VP15tr(aa1–25)-RV | GGAGGAGCGAGCCACCATCTTCAG |
VP15(58–80) | |
VP15tr(aa58–80)-FW | AAGTCCTCCACCGTGCGT |
pGEX-InvRV-ATG | CATGGATCCCAGGGGC |
VP15(1–25, 58–80) | |
VP15tr(aa58–80)-FW | AAGTCCTCCACCGTGCGT |
VP15tr(aa1–25)-RV | GGAGGAGCGAGCCACCATCTTCAG |
VP15(26–57) | |
pGEX-InvFW-Flag | GACTACAAGGATGACGATGACAAGTAAG |
VP15tr(aa26–57)-RV | TTTGCCAGCGCGCTTCTT |
VP15(SR–11) | |
pGEX-InvFW-ISKRR-FLAG | ATCTCCAAGCGTCGTGACTACAAGGATGACGATGACAAG |
pGEX-InvRV-STTAGR | GCGGCCAGCGGTGGTGGACATGGATCCCAGGGGCCCCT |
MjgC1qR | |
MjgC1qR-FW-BamHI | ACCAGGATCCATGAGTGCCATCAGTCGTGC |
MjgC1qR-RV-XhoI-NoStop | TTGTCTCGAGTTTCCTCTTGACAAAGTCCT |
