Table 1 Purification steps of Apr-BO1.

From: Isolation and characterization of a new cold-active protease from psychrotrophic bacteria of Western Himalayan glacial soil

Purification

Total activity (U)

Total protein (mg)

Specific activity (U/mg)

Purification folds

Culture filtrate

92.4 ± 7.6

229.3 ± 26.73

0.40

1

Ammonium sulphate precipitation

316.80 ± 19.28

178.6 ± 14.05

1.77

4.43

Dialyzed fraction

789.12 ± 18.19

112.0 ± 2.68

7.05

17.63

Sephadex G-50

1085.76 ± 34.5

29.33 ± 0.61

37.02

92.55

  1. The values are the mean of three replicates ± standard deviation.
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