Table 2 Effect of metal ions and inhibitor on the activity of purified cold-active Apr-BO1 at three different concentrations.

From: Isolation and characterization of a new cold-active protease from psychrotrophic bacteria of Western Himalayan glacial soil

Metal ions

1.0 mM (%)

5.0 mM (%)

10.0 mM (%)

Control

100 ± 0.0

100 ± 0.0

100 ± 0.0

Mn2+

103.50 ± 2.2

114.00 ± 0.3

115.94 ± 0.1

Mg2+

100 ± 1.0

112.3 ± 0.92

100.67 ± 2.1

Hg2+

59.58 ± 0.6

16.03 ± 0.6

3.90 ± 0.5

Cd2+

63.07 ± 0.9

60.71 ± 0.5

30.40 ± 1.0

Co2+

81.26 ± 1.1

23.34 ± 2.9

5.37 ± 0.7

Fe2+

113.37 ± 0.8

100.35 ± 0.7

99.71 ± 0.6

Cu2+

108.92 ± 1.1

106.22 ± 1.4

96.03 ± 0.8

Zn2+

106.42 ± 0.8

105.09 ± 0.9

51.58 ± 3.6

Ca2+

112.53 ± 1.0

119.04 ± 2.2

123.69 ± 1.6

EDTA

77.7 ± 0.7

35.17 ± 0.7

11.5 ± 1.3

  1. The values are the mean of three replicates ± standard deviation.