Figure 5

FVIII binding sites and neighboring residues. (a) Location of known FVIII binding sites (pink) and their immediate neighboring residues (blue) in the FVIII structure and in the RIN. Image created using the structure 2R7E (Ref.³) and Chimera 1.14 (Ref.⁶²). (b) Comparison of the centrality measures of the residues reported to be part of a binding site and their immediate neighboring amino acids. The boxplots depict the median (center line), the first and third quartiles (lower- and upper-bounds), and 1.5 times the inter-quartile range (lower- and upper whiskers). Each dot in the plot is a node from the FVIII RIN (i.e., an amino acid). Unpaired, two-sided Wilcoxon test (***Indicate p-values < 0.001).