Figure 6

IHZ ameliorates hepatic fibrosis, morphological changes and adipose tissue alterations in diabetic rats by the inhibition of JNK signaling pathway. To detect morphological changes H&E staining was performed (a, black arrow shows change in morphology); Picro-Sirius red staining was performed to assess the extent of fibrosis and collagen deposition (Red color indicated depositions of collagen) (b); PKR expression in adipose and liver tissue was determined by immunohistochemistry using anti-PKR antibody (c); Graphs representing the quantitative data of the staining assays (d–g). Representative western blots showing protein levels of PKR and JNK in liver tissue of different treatment groups (h) and the quantitative analysis of these blots (i,j). Data is presented as mean ± SD of n = 3 animals for each group. ***P < 0.001 vs control group; %P < 0.05, %%P < 0.01 vs HF + STZ group.