Figure 5

Cortex of a cervical lymph node. Immunohistochemical co-staining with polyclonal anti-GFAP antibody (DAKO) in red (directly conjugated) and anti-CD31 in orange, a marker for blood endothelium. DAPI for nuclear staining in blue. Colocalization between GFAP and CD31 is highlighted by arrows. Colocalization is clear but incomplete. GFAP immunoreactivity is also clearly visible in the parenchyma around the vessel (arrowheads), while CD31 is limited to the blood vessel (asterix).