Figure 2
From: Enrichment of low abundance DNA/RNA by oligonucleotide-clicked iron oxide nanoparticles
InBeads evaluation with synthetic HCV targets. Capture specificity was evaluated with a synthetic DNA gblock representing two adjacent regions of the HCV genome (A and B), mixed with 100 ng of human genomic DNA to simulate a clinical sample background. Copy number was measured by real-time qPCR, for probes A and B as well as for a human housekeeping gene B2M, both before (a) and after (b) capture. Notably, B2M copy numbers were above-range before capture and undetectable after capture. (c) Enrichment factor is the ratio of copy numbers before and after capture.
