Figure 6 | Scientific Reports

Figure 6

From: Differences in in vitro microglial accumulation of the energy metabolism tracers [18F]FDG and [18F]BCPP-EF during LPS- and IL4 stimulation

Figure 6

Putative changes in [18F]FDG and [18F]BCPP-EF uptake and metabolic profiles in LPS- or IL4-stimulated microglia. In LPS-stimulated microglial cells, overexpression of glycolysis-related genes (GLUT1 and HK2) was observed in the present study, and glycolysis and oxidative phosphorylation (OXPHOS) activity were previously reported to be increased and decreased, respectively. [14C]2DG ([18F]FDG) and [18F]BCPP-EF uptake were increased and decreased according to these changes in metabolic profiles, respectively, and the [18F]BCPP-EF/[14C]2DG ([18F]BCPP-EF/[18F]FDG) ratio was decreased. In IL4-stimulated microglial cells, glycolysis and OXPHOS activity were speculated to be decreased and increased, respectively, without a change in gene expression. [14C]2DG ([18F]FDG) and [18F]BCPP-EF uptake was decreased and increased according to previously reported changes in metabolic activity profiles, respectively, and the [18F]BCPP-EF/[14C]DG ([18F]BCPP-EF/[18F]FDG) ratio was increased. The red and blue arrows indicate increased and decreased expression, activity, or uptake, respectively.

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