Figure 1
From: SIRT2 promotes murine melanoma progression through natural killer cell inhibition
Systemic SIRT2 overexpression increases growth of melanoma tumors in mice. (a) Gross specimens of subcutaneous melanoma tumors harvested from Sirt2-KI and WT mice inoculated with 1 × 105 B16-F10 melanoma cells. (b) Tumors in WT (n = 14) and Sirt2-KI (n = 16) mice were harvested on Day 20 and weighed. Student’s t test. (c) Tumor progression and size were monitored 20 days post injection in WT and Sirt2-KI mice (n = 3). Two-way ANOVA with Bonferroni correction. (d) Western blot showing decreased SIRT2 expression in Sirt2-KO B16-F10 cells following Sirt2 knockout via CRISPR gene editing. Sirt2-OE B16-F10 cells overexpress SIRT2 as demonstrated by western blot following transfection with Flag-WT-Sirt2. (e) C57BL/6 WT mice were injected with WT, Sirt2-OE, and Sirt2-KO B16-F10 melanoma cells and tumor development and growth were monitored for 14 days. No significant difference in tumor progression was observed between the cell groups. n = 10. Two-way ANOVA with post-hoc Bonferroni correction. Data points are mean values ± SEM.*P < 0.05 and ****P < 0.0001.
