Figure 2
From: SIRT2 promotes murine melanoma progression through natural killer cell inhibition
Natural killer cell distribution is altered by SIRT2 expression. (a) Subcutaneous melanoma tumors from WT and Sirt2-KI mice were harvested and analyzed by flow cytometry for the prevalence of NK cells among leukocytes. The gating strategy was designed to isolate NK cells by selecting for cells that are NKp46 positive and CD3E negative. (b) The percentage of tumor-infiltrating leukocytes that expressed the NK cell signature were quantified in WT (n = 22) and Sirt2-KI mice (n = 11). (c) Absolute quantities of NK cells are illustrated in Sirt2-KI and WT mice (n = 6). Data points represent mean values with error bars representing the SEM. Student’s t test,*P < 0.05 and ***P < 0.001.
