Figure 1
Assessment of MB antiviral activity on human H1N1. MB was incubated with H1N1 virus A/Netherlands/602/2009 (1.5 × 107 pfu) for 2 h or 20 h in the darkness (a, c) or in the presence of light (b, d). At the end of the incubation, mixtures were serially diluted and added for 1 h at 37 °C on MDCK cells (a) and (b). Mixtures were then removed and the cells were overlaid with medium containing 0.8% agarose and TPCK trypsin 1 µg/ml. At 48 h post infection (hpi) the cells were fixed in order to count the plaques and determine the viral titer in each experimental condition. In parallel, viral RNA was quantified by RT-qPCR (c, d) and viral genome integrity was assessed by PCR amplification of the NP (e) and M (f) viral segments. In (e) and (f) only the “UT” and “MB 2 µg/ml” conditions were examined. Results are mean and SD of two independent experiments. 2 µg/ml (6.25 µM), 0.4 µg/ml (1.25 µM), 0.08 µg/ml (0.25 µM). UT = untreated. **p < 0.01, ***p < 0.001, ****p < 0.0001.
