Figure 3
Subcellular localisation of CFAP161. (A) Indirect immunofluorenscence staining of murine adult fallopian tube sections (a, b), sections of adult respiratory epithelium of the nasal cavity (c, d), and seminiferous tubule of the adult testis (e, e′) showing localisation of CFAP161 to the ciliary axoneme and flagella, respectively. The distal region of cilia marked by white arrowhead lacks CFAP161. Boxed areas in a-d indicate the regions shown at higher magnification in a′–d′ and a″–d″, boxed areas in a′–d′ and a″–d″ indicate the regions shown enlarged in the respective insets. (B) Localisation of mouse GPF-CFAP161 in Xenopus MCCs co-expressing the basal body marker Cetn4-RFP (a) revealed accumulation of CFAP161 in anterior juxtaposition (a′) underneath Cetn4 (a″) as illustrated in (b). Boxed area in (a) indicate the region shown at higher magnification in (a′). Orthogonal section as indicated and shown in (a″). Scale bars: (A)a–d = 100 µm; (A)e, e′ = 25 µm; B = 100 µm.
