Figure 4
No cilia related phenotypes in Xenopus cfap161-crispants. (A) Representative wild type (a) and crispant (b) tadpoles at stage 45. (B) Evaluation of organ situs, embryonic cysts (C) and hydrocephalus (D) in 297 crispants obtained in 5 independent experiments. Statistical calculation of organ situs defects, cyst analysis or hydrocephalus formation was performed using chi square (http://www.physics.csbsju.edu/stats/contingency_NROW_NCOLUMN_form.html). (E) Ciliary beat frequency (CBF) in wild type and crispants. (a) Statistical evaluation of CBF. Results from 3 independent experiments with each 5 embryos and 5 analysed MCCs per embryo. Graph displays respective values with mean and s.d. Raw data are shown in Table S3. (b) Kymographs of ciliary motility of single MCCs of wild type and crispant embryos. (F) Cilia generated flow (CGF) (a) Statistical evaluation of CGF, velocities of bead transport in wild type and crispant embryos. Results from 3 independent experiments with 8 analysed specimens each. Graph displays respective values with mean and s.d. Raw data are shown in Table S4. (b) Maximum intensity projection of bead transport of single wild type and crispant embryo. Compared to wild type specimens no significant deviations were recorded in any experiment. ns P > 0.05. p-values were calculated via Wilcoxon-Match-Pair test in RStudio. Scale bar: (A) = 500 µm.
