Figure 4 | Scientific Reports

Figure 4

From: Genetic engineering of porcine endothelial cell lines for evaluation of human-to-pig xenoreactive immune responses

Figure 4

Characterization of primary pLDEC and immortalized porcine liver-derived endothelial cells (ipLDEC). Porcine cells were analyzed for endothelial cell markers, including cell surface expression of CD31, intracellular expression of von Willebrand factor (VWF), and E-selectin (rhTNF-α stimulation) by flow cytometry. SLA-I and SLA-II expression in porcine cells before and after porcine IFN-γ stimulation (pIFN-γ) were examined by flow cytometric analysis using specific antibodies. Primary pLDEC (passage 15–20) and ipLDEC (passage 20–30 after immortalization) were used for characterization.

Back to article page