Table 2 PCR primers used to amplify gRNA targeting regions.

From: Genetic engineering of porcine endothelial cell lines for evaluation of human-to-pig xenoreactive immune responses

Gene

PCR primers

Amplicon (bp)

GGTA1

CCTTAGTATCCTTCCCAACCCAGAC

GCTTTCTTTACGGTGTCAGTGAATCC

428

CMAH

CTTGGAGGTGATTTGAGTTGGG

CATTTTCTTCGGAGTTGAGGGC

458

β4galNT2

CGCAAGTGACCAGACATCGTTC

AAAGCCACAGGAGGAGCCAG

530

B2M

TCTTTCTAACCTGCTCGGGC

CGATCTGAAGCTTACCCGCA

530

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