Figure 6
From: Photobiomodulation has rejuvenating effects on aged bone marrow mesenchymal stem cells

Effects of single and multiple doses of PBM on proliferation of young and aged MSCs: Both young and aged MSCs underwent a PBM treatment at 3 J/cm2 for 1, 2 or 3 days in a row. The control group did not receive any treatment. (A) On day 7, the cells from both the control and the experimental groups were harvested to determine the cell viability and total number of cells in each group using a trypan blue exclusion assay. Shown is the percentage of cell proliferation in each group relative to the untreated young MSC control group, with significant differences indicated by asterisk signs. *p < 0.05; **p < 0.01. (B) On day 7, metabolic activity was measured using WST-1 assays. Shown is the percentage of cell proliferation in each group relative to the untreated young MSC control group with significant differences indicated by asterisk signs. **p < 0.01; ****p < 0.0001.