Table 1 Primer list.
Gene | Forward primer | Reverse primer | Amplicon size (bp) |
|---|---|---|---|
Gapdh | GCTCTCTGCTCCTCCTGTTC | GTTGACTCCGACCTTCACCT | 103 |
Nanog | GTCCCGGTCAAGAAACAGAA | GCTGAGGTTCAGGATGTTGG | 135 |
Oct4 | CCCGAAAGAGAAAGCGAAC | CACTCGGACCACATCCTTCT | 143 |
Sox2 | GCCGAGTGGAAACTTTTGTC | GGGCAGCGTGTACTTATCCTT | 192 |
Runx2 | CCGTCCATCCACTCTACCAC | CCAGAGGCAGAAGTCAGAGG | 120 |
Alpl | GGAGATGGGATGGGTGTCT | CCACGAAGGGGAACTTGTC | 112 |
Col1a1 | GACGAAGACATCCCACCAAT | CATCGCACAACACCTTGC | 121 |
Opn | GCCGAGGTGATAGTGTGGTT | GCTTTCCATGTGTGAGGTGAT | 113 |
Phex | GACTTTGCTGAGGGAGAGCA | CGCCACCCTTTCTCAAATAA | 132 |
Sost | CAGCCTTCCGTGTAGTGGAG | CGGTTCATGGTCTTGTTGTTC | 127 |
Sox9 | TGGAGACTTCTGAACGAGAGC | CGTTCTTCACCGACTTCCTC | 127 |
Acan | ACTCGCTGACCTTTGAGGAG | GGCTCACAATGGGGTATCTG | 156 |
Col10a1 | CTTCTGCACTGCTCATCTGG | GTGGGCATTTGGTATCGTTC | 179 |
Mmp13 | GCTGCCTTCCTCTTCTTGAG | CTCAGTCATGGAGCTTGCTG | 180 |
Pparg | GAAGGATGCAAGGGTTTCTTC | CCAACAGCTTCTCCTTCTCG | 199 |
Cebpa | GGGACCGGAGTTATGACAAG | GGCAAGTATCCGAGCAAAAC | 174 |
