Figure 6
From: Progerin impairs 3D genome organization and induces fragile telomeres by limiting the dNTP pools
Progerin expression limits dNTPs accessibility. (A) Percentage of EdU positive cells in HDFs expressing NLS-EGFP (N), EGFP-LA (A), and EGFP-PG (P). When indicated, cells were supplemented with dNTPs for 12 or 24 h, then pulse-labeled with EdU for 30 min. Mean with SD is shown, n = 3. Statistical significance was determined using one-way Anova (**indicates p < 0.001). (B) Percentage of EdU positive cells in HDF and HGPS cells with (right) or without (left) hTERT expression. When indicated, cells were supplemented with dNTPs for 24 h, then pulse-labeled with EdU for 30 min. Mean with SD is shown, each dot corresponding to a replicate (n = 2 to n = 6 depending of the cell line). Statistical significance was determined using one-way Anova (*indicates p < 0.005). (C) Western blot of total extracts extracted from the indicated cell lines. When indicated, cells were supplemented with dNTPs for 24 h before collecting. (D) Quantification of MTS in HDF expressing the indicated constructs. The graphs represent the number of MTS per metaphase. Mean with SD is shown, n = 3. At least 30 metaphases were scored per condition in total. Statistical significance was determined using Mann Whitney test (***indicates p < 0.0005, and ****p < 0.0001).
