Figure 7 | Scientific Reports

Figure 7

From: MCT4 is induced by metastasis-enhancing pathogenic mitochondrial NADH dehydrogenase gene mutations and can be a therapeutic target

Figure 7

Analyses of signalling pathways involved in MCT4 expression in P29mtB82M and H358 cells by signalling pathway inhibitors. (a) Effects of various inhibitors on MCT4 expression in P29mtB82M cells. The cells were treated with the inhibitors at the indicated concentrations for 2 days. (b–e) Effect of LY294002, rapamycin, BML-275 and PF-4709671 on the expressions of MCT4 and MCT1. P29mtB82M cells were treated with LY294002 (b), rapamycin (c), BML-275 (d) or PF-4709671 (e) at the indicated concentrations for 2 days. The results of RT-qPCR and Western blotting are shown. (f) Comparison of phosphorylation levels of various signalling elements in P29mtP29 and P29mtB82M cells. Ratios of phosphorylated/total protein (p/t) are shown. (g) Effects of various inhibitors on MCT4 expression in H358 cells. Cells were treated with the inhibitors at the indicated concentrations for 2 days. For Western blotting, β-Actin was used as a loading control. Ratios of MCT4/β-actin are shown. Uncropped Western blot images are provided as Supplementary Fig. S12.

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