Figure 1

Comparing the serum AP3D1-antibody levels between healthy donors (HDs) and patients with AIS and TIA. This figure shows the levels of serum antibodies against AP3D1 (s-AP3D1-Abs) examined using amplified luminescence proximity homogeneous assay-linked immunosorbent assay (AlphaLISA) (a). The bars represent the average and average ± standard deviation (SD). P-values were calculated using the Kruskal–Wallis test (Mann–Whitney U test with Bonferroni's correction applied). ***P < 0.001. The total average values, SDs, cutoff values, positive numbers, positive rates (%), and P-values are summarized in Table 1. A receiver operating characteristic (ROC) curve analysis was performed to assess the abilities of s-AP3D1-Abs in detecting either (b) acute ischemic stroke (AIS) or (c) transient ischemic attack (TIA). The numbers in the figures indicate the cutoff values for marker levels, and the numbers in parentheses indicate sensitivity (left) and specificity (right). P value, area under the curve (AUC), and 95% confidence intervals (95% CI) are also shown. AP3D1, adaptor-related protein complex 3 subunit delta 1. The results of the power calculation using G-Power 3.1 software were 0.9762618 for AIS and 0.9953412 for TIA (Supplementary Table S3).