Figure 1
From: Porcine pancreatic ductal epithelial cells transformed with KRASG12D and SV40T are tumorigenic
Isolation and transduction of primary porcine pancreatic ductal epithelial cells. (A) Immunofluorescent staining for Pan-Keratin and cytokeratin 19 (CK-19) in cultured primary epithelial cells. (B) Immunoblot for SV40T, mutant KRASG12D, and Pan-Keratin in primary epithelial cells, SV40T immortalized cells, and SV40T immortalized cells transformed with KRASG12D (SV40T LV-GK; two lines, CL1 and CL2) Samples are from the same gel/blot with different IR Dye exposures depending on the secondary for the primary antibody. For full blot images see Fig. S3. Black arrow = band of interest; MWM = molecular weight marker. (C) Expression of ZsGreen1 (a green fluorescent protein variant) in primary epithelial cells and SV40T immortalized cells transduced with the LV-GK; phase and inverted fluorescent microscopy of living cells. Measure bars = 400 µm.
