Figure 2
From: Porcine pancreatic ductal epithelial cells transformed with KRASG12D and SV40T are tumorigenic
In vitro cell transformation assays. Cultured primary epithelial cells from porcine pancreatic duct were compared with SV40T immortalized and transformed (SV40T + KRASG12D) cell lines. (A) Cell culture population doubling time (count-based analysis). (B) Proliferation rate using metabolic dye assay, represented as fold change with respect to primary cells (defined as 1). (C) Phase contrast images of soft agar assay (bar = 1000 µm). (D) Plot of soft agar assay. (E–F) Transwell migration assay (absence of BME), phase images and plot. (G–H) Transwell invasion assay (presence of BME), phase images and plot. Each bar or data point in this Figure represents the mean ± SD of triplicate wells; each experiment performed three times on separate days (one representative experiment shown in each panel); ****p < 0.0001; ns = not significant (p ≥ 0.05).
