Figure 3
From: Porcine pancreatic ductal epithelial cells transformed with KRASG12D and SV40T are tumorigenic
In vivo tumorigenesis assay. (A) Representative xenograft tumor explanted from a nude mouse 42 days after subcutaneous injection of transformed epithelial cells (SV40T LV-GK CL2). (B) Comparison of xenograft tumor volume between primary epithelial cells versus the SV40T immortalized and SV40T LV-GK CL2 cell lines; ****p < 0.0001; ns = not significant (p ≥ 0.05). (C) H&E microscopic image of xenograft tumor. (D) Higher power H&E image; region of interest box shown in panel C. Black arrows = hyperchromatic nuclei; yellow arrows = nuclei with large nucleoli; red arrows = pyknotic nuclei. (E) Higher power H&E image; region of interest box shown in panel D. *Asterisks indicate lumen of gland-like structures lined with ductal-like cells; lumen were filled with mucin in the Alcian blue staining (Fig. S6).
