Figure 4
From: Porcine pancreatic ductal epithelial cells transformed with KRASG12D and SV40T are tumorigenic
Immunohistochemistry of tumor xenografts from subcutaneous nude mouse assay. (A) Pan-Keratin; section from human PDAC (original tumor, not a xenograft); utilized as a positive control (nuclear counterstain = hematoxylin). Panels B-G are all from xenograft tumor, obtained with implantation of the SV40T LV-GK CL2 cell line. (B) Negative control (all reagents except no primary antibody). Black arrows = ductal-like structures; dashed yellow circles = acinar-like structures. (C) Pan-Keratin. (D) KRASG12D (antibody specific to mutant KRAS). (E) SV40T. (F) Ki-67. (G) p21.
